3D-localization microscopy and tracking of FoF1-ATP synthases in living bacteria
Autor(en): | Renz, A. Renz, M. Klütsch, D. Deckers-Hebestreit, G. Börsch, M. |
Herausgeber: | Gregor, I. Gryczynski, Z.K. Koberling, F. Enderlein, J. Erdmann, R. |
Stichwörter: | Adenosinetriphosphate; Biosynthesis; Diffusion; Escherichia coli; FoF1-ATP synthase; Mean squared displacement; Molecules; Observation window; Optical resolving power, ATP synthase; PALM; Physiological condition; Single particle tracking; Structured illumination microscopies (SIM); Super resolution imaging, Palmprint recognition; Superresolution imaging | Erscheinungsdatum: | 2015 | Herausgeber: | SPIE | Journal: | Progress in Biomedical Optics and Imaging - Proceedings of SPIE | Volumen: | 9331 | Zusammenfassung: | FoF1-ATP synthases are membrane-embedded protein machines that catalyze the synthesis of adenosine triphosphate. Using photoactivation-based localization microscopy (PALM) in TIR-illumination as well as structured illumination microscopy (SIM), we explore the spatial distribution and track single FoF1-ATP synthases in living E. coli cells under physiological conditions at different temperatures. For quantitative diffusion analysis by mean-squared-displacement measurements, the limited size of the observation area in the membrane with its significant membrane curvature has to be considered. Therefore, we applied a 'sliding observation window' approach (M. Renz et al., Proc. SPIE 8225, 2012) and obtained the one-dimensional diffusion coefficient of FoF1-ATP synthase diffusing on the long axis in living E. coli cells. © 2015 SPIE. |
Beschreibung: | Conference of Single Molecule Spectroscopy and Superresolution Imaging VIII ; Conference Date: 7 February 2015 Through 8 February 2015; Conference Code:112031 |
ISBN: | 9781628414219 | ISSN: | 16057422 | DOI: | 10.1117/12.2080981 | Externe URL: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84930463793&doi=10.1117%2f12.2080981&partnerID=40&md5=c7e01ee6a4c576f2c83516db05dae065 |
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