Elucidating the biosynthesis of chitin filaments and their configuration with specific proteins and electron microscopy
Autor(en): | Siemieniewicz, Krzysztof W. Kajla, Mayur K. Schrempf, Hildgund |
Stichwörter: | ALPHA-CHITIN; BINDING; Biochemistry & Molecular Biology; CHB1; CHITOSOMES; FUNGI; GENE; GROWTH; LOCALIZATION; Materials Science; Materials Science, Biomaterials; Polymer Science; SYNTHETASE | Erscheinungsdatum: | 2007 | Herausgeber: | WILEY-V C H VERLAG GMBH | Enthalten in: | MACROMOLECULAR BIOSCIENCE | Band: | 7 | Ausgabe: | 1 | Startseite: | 40 | Seitenende: | 47 | Zusammenfassung: | To deepen the knowledge of chitin synthesis, a yeast mutant has been used as a model. Purified chitin synthase I-containing vesicles (chitosomes) with a diameter of 85 to 120 nm are identified by electron microscopy to eject tiny fibers upon addition of UDP-N-acetylglucosamine. The filigree of extruded filaments fused gradually into a large three-dimensional network, which is degradable by a chitinase. The network is targeted and restructured by the Streptomyces chitin-binding protein CHB1, which has a very high affinity only for alpha-chitin. Within the chitosomes, filaments are found to be highly condensed within consecutive oval fibroids, which are specifically targeted by the alpha-chitin-binding protein. The presented data give new insights to the generation of chitin filaments with an antiparallel (alpha) configuration. |
ISSN: | 16165187 | DOI: | 10.1002/mabi.200600180 |
Zur Langanzeige
Seitenaufrufe
2
Letzte Woche
0
0
Letzter Monat
0
0
geprüft am 06.06.2024