Elucidating the biosynthesis of chitin filaments and their configuration with specific proteins and electron microscopy

Autor(en): Siemieniewicz, Krzysztof W.
Kajla, Mayur K.
Schrempf, Hildgund 
Stichwörter: ALPHA-CHITIN; BINDING; Biochemistry & Molecular Biology; CHB1; CHITOSOMES; FUNGI; GENE; GROWTH; LOCALIZATION; Materials Science; Materials Science, Biomaterials; Polymer Science; SYNTHETASE
Erscheinungsdatum: 2007
Herausgeber: WILEY-V C H VERLAG GMBH
Journal: MACROMOLECULAR BIOSCIENCE
Volumen: 7
Ausgabe: 1
Startseite: 40
Seitenende: 47
Zusammenfassung: 
To deepen the knowledge of chitin synthesis, a yeast mutant has been used as a model. Purified chitin synthase I-containing vesicles (chitosomes) with a diameter of 85 to 120 nm are identified by electron microscopy to eject tiny fibers upon addition of UDP-N-acetylglucosamine. The filigree of extruded filaments fused gradually into a large three-dimensional network, which is degradable by a chitinase. The network is targeted and restructured by the Streptomyces chitin-binding protein CHB1, which has a very high affinity only for alpha-chitin. Within the chitosomes, filaments are found to be highly condensed within consecutive oval fibroids, which are specifically targeted by the alpha-chitin-binding protein. The presented data give new insights to the generation of chitin filaments with an antiparallel (alpha) configuration.
ISSN: 16165187
DOI: 10.1002/mabi.200600180

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