Self-assembly of robust gold nanoparticle monolayer architectures for quantitative protein interaction analysis by LSPR spectroscopy

Autor(en): Flesch, Julia
Kappen, Marie
Drees, Christoph
You, Changjiang 
Piehler, Jacob 
Stichwörter: Biochemical Research Methods; Biochemistry & Molecular Biology; Chemistry; Chemistry, Analytical; COPOLYMERS; DYNAMICS; HOT-SPOTS; Kinetics; Localized surface plasmon resonance (LSPR); Protein immobilization; Quantitative interaction analysis; REAL-TIME; Real-time biosensor; RECEPTOR INTERACTIONS; REVEALS; Self-assembly; SERS; SINGLE-MOLECULE; TAGGED PROTEINS; TRACKING
Erscheinungsdatum: 2020
Herausgeber: SPRINGER HEIDELBERG
Journal: ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volumen: 412
Ausgabe: 14, SI
Startseite: 3413
Seitenende: 3422
Zusammenfassung: 
Localized surface plasmon resonance (LSPR) detection offers highly sensitive label-free detection of biomolecular interactions. Simple and robust surface architectures compatible with real-time detection in a flow-through system are required for broad application in quantitative interaction analysis. Here, we established self-assembly of a functionalized gold nanoparticle (AuNP) monolayer on a glass substrate for stable, yet reversible immobilization of Histidine-tagged proteins. To this end, one-step coating of glass substrates with poly-L-lysine graft poly(ethylene glycol) functionalized with ortho-pyridyl disulfide (PLL-PEG-OPSS) was employed as a reactive, yet biocompatible monolayer to self-assemble AuNP into a LSPR active monolayer. Site-specific, reversible immobilization of His-tagged proteins was accomplished by coating the AuNP monolayer with tris-nitrilotriacetic acid (trisNTA) PEG disulfide. LSPR spectroscopy detection of protein binding on these biocompatible functionalized AuNP monolayers confirms high stability under various harsh analytical conditions. These features were successfully employed to demonstrate unbiased kinetic analysis of cytokine-receptor interactions.
ISSN: 16182642
DOI: 10.1007/s00216-020-02551-6

Show full item record

Page view(s)

2
Last Week
0
Last month
0
checked on Mar 4, 2024

Google ScholarTM

Check

Altmetric