Live cell imaging of cytoskeletal dynamics in neurons using fluorescence photoactivation

Autor(en): Gauthier, Anne
Brandt, Roland 
Stichwörter: Alzheimer's disease; Biochemistry & Molecular Biology; DEGENERATION; EXPRESSION; microtubule-associated protein; neurodegeneration; photoactivatable GFP; photoactivation; PROTEINS; SCLEROSIS; TAU; tau protein; TRANSPORT
Erscheinungsdatum: 2010
Herausgeber: WALTER DE GRUYTER GMBH
Journal: BIOLOGICAL CHEMISTRY
Volumen: 391
Ausgabe: 6
Startseite: 639
Seitenende: 643
Zusammenfassung: 
Alzheimer's disease and other neurodegenerative disorders share some common features at the cellular level, which are often associated with a change in cytoskeletal dynamics. Live cell imaging has been applied to study various aspects of cell physiology including cytoskeletal dynamics. Recently, fluorescence photoactivation (FPA) has been developed as a novel approach to analyze protein dynamics in living cells with high sensitivity. Here, we describe the application of HA to determine interactions and mobility of the microtubule-associated protein tau in neurons as an example for a disease-related cytoskeletal protein and discuss further applications of FPA to study cytoskeletal dynamics in neurodegenerative diseases.
ISSN: 14316730
DOI: 10.1515/BC.2010.060

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