Live cell imaging of cytoskeletal dynamics in neurons using fluorescence photoactivation
Autor(en): | Gauthier, Anne Brandt, Roland |
Stichwörter: | Alzheimer's disease; Biochemistry & Molecular Biology; DEGENERATION; EXPRESSION; microtubule-associated protein; neurodegeneration; photoactivatable GFP; photoactivation; PROTEINS; SCLEROSIS; TAU; tau protein; TRANSPORT | Erscheinungsdatum: | 2010 | Herausgeber: | WALTER DE GRUYTER GMBH | Journal: | BIOLOGICAL CHEMISTRY | Volumen: | 391 | Ausgabe: | 6 | Startseite: | 639 | Seitenende: | 643 | Zusammenfassung: | Alzheimer's disease and other neurodegenerative disorders share some common features at the cellular level, which are often associated with a change in cytoskeletal dynamics. Live cell imaging has been applied to study various aspects of cell physiology including cytoskeletal dynamics. Recently, fluorescence photoactivation (FPA) has been developed as a novel approach to analyze protein dynamics in living cells with high sensitivity. Here, we describe the application of HA to determine interactions and mobility of the microtubule-associated protein tau in neurons as an example for a disease-related cytoskeletal protein and discuss further applications of FPA to study cytoskeletal dynamics in neurodegenerative diseases. |
ISSN: | 14316730 | DOI: | 10.1515/BC.2010.060 |
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