Diffusion and Interaction Dynamics of Individual Membrane Protein Complexes Confined in Micropatterned Polymer-Supported Membranes

DC FieldValueLanguage
dc.contributor.authorWaichman, Sharon
dc.contributor.authorRoder, Friedrich
dc.contributor.authorRichter, Christian P.
dc.contributor.authorBirkholz, Oliver
dc.contributor.authorPiehler, Jacob
dc.date.accessioned2021-12-23T16:01:36Z-
dc.date.available2021-12-23T16:01:36Z-
dc.date.issued2013
dc.identifier.issn16136810
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/5054-
dc.description.abstractMicropatterned polymer-supported membranes (PSM) are established as a tool for confining the diffusion of transmembrane proteins for single molecule studies. To this end, a photochemical surface modification with hydrophobic tethers on a PEG polymer brush is implemented for capturing of lipid vesicles and subsequent fusion. Formation of contiguous membranes within micropatterns is confirmed by scanning force microscopy, fluorescence recovery after photobleaching (FRAP), and super-resolved single-molecule tracking and localization microscopy. Free diffusion of transmembrane proteins reconstituted into micropatterned PSM is demonstrated by FRAP and by single-molecule tracking. By exploiting the confinement of diffusion within micropatterned PSM, the diffusion and interaction dynamics of individual transmembrane receptors are quantitatively resolved.
dc.description.sponsorshipDFGGerman Research Foundation (DFG)European Commission [PI405-4, SFB 944]; European CommunityEuropean Commission [223608]; FP6 Marie-Curie EST fellowship [MEST-CT-2004-504272]; Minerva Foundation; S.W. and F.R. contributed equally to this manuscript. We thank Gabriele Hikade and Hella Kenneweg for technical support. This project was supported by funding from the DFG (PI405-4 and SFB 944) and by the European Community's Seventh Framework Programme (FP7/2007-2013) under grant agreement no 223608 (IFNaction). S.W. was supported by a FP6 Marie-Curie EST fellowship (grant no. MEST-CT-2004-504272) and by a PhD fellowship from the Minerva Foundation.
dc.language.isoen
dc.publisherWILEY-V C H VERLAG GMBH
dc.relation.ispartofSMALL
dc.subjectBILAYERS
dc.subjectCELL-MEMBRANES
dc.subjectChemistry
dc.subjectChemistry, Multidisciplinary
dc.subjectChemistry, Physical
dc.subjectGIANT UNILAMELLAR VESICLES
dc.subjectIMMOBILIZATION
dc.subjectLATERAL DIFFUSION
dc.subjectLIPID-MEMBRANES
dc.subjectMaterials Science
dc.subjectMaterials Science, Multidisciplinary
dc.subjectmembrane proteins
dc.subjectMICROSCOPY
dc.subjectNanoscience & Nanotechnology
dc.subjectphotolithography
dc.subjectPhysics
dc.subjectPhysics, Applied
dc.subjectPhysics, Condensed Matter
dc.subjectpolymer-supported membranes
dc.subjectproteinprotein interactions
dc.subjectRECEPTOR INTERACTIONS
dc.subjectScience & Technology - Other Topics
dc.subjectSINGLE-MOLECULE FLUORESCENCE
dc.subjectsingle-molecule imaging
dc.subjectTRACKING
dc.titleDiffusion and Interaction Dynamics of Individual Membrane Protein Complexes Confined in Micropatterned Polymer-Supported Membranes
dc.typejournal article
dc.identifier.doi10.1002/smll.201201530
dc.identifier.isiISI:000315103300011
dc.description.volume9
dc.description.issue4
dc.description.startpage570
dc.description.endpage577
dc.contributor.orcid0000-0001-6551-3219
dc.identifier.eissn16136829
dc.publisher.placePOSTFACH 101161, 69451 WEINHEIM, GERMANY
dcterms.isPartOf.abbreviationSmall
crisitem.author.deptFB 05 - Biologie/Chemie-
crisitem.author.deptidfb05-
crisitem.author.orcid0000-0002-2143-2270-
crisitem.author.parentorgUniversität Osnabrück-
crisitem.author.netidPiJa938-
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