Diffusion and Interaction Dynamics of Individual Membrane Protein Complexes Confined in Micropatterned Polymer-Supported Membranes
DC Element | Wert | Sprache |
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dc.contributor.author | Waichman, Sharon | |
dc.contributor.author | Roder, Friedrich | |
dc.contributor.author | Richter, Christian P. | |
dc.contributor.author | Birkholz, Oliver | |
dc.contributor.author | Piehler, Jacob | |
dc.date.accessioned | 2021-12-23T16:01:36Z | - |
dc.date.available | 2021-12-23T16:01:36Z | - |
dc.date.issued | 2013 | |
dc.identifier.issn | 16136810 | |
dc.identifier.uri | https://osnascholar.ub.uni-osnabrueck.de/handle/unios/5054 | - |
dc.description.abstract | Micropatterned polymer-supported membranes (PSM) are established as a tool for confining the diffusion of transmembrane proteins for single molecule studies. To this end, a photochemical surface modification with hydrophobic tethers on a PEG polymer brush is implemented for capturing of lipid vesicles and subsequent fusion. Formation of contiguous membranes within micropatterns is confirmed by scanning force microscopy, fluorescence recovery after photobleaching (FRAP), and super-resolved single-molecule tracking and localization microscopy. Free diffusion of transmembrane proteins reconstituted into micropatterned PSM is demonstrated by FRAP and by single-molecule tracking. By exploiting the confinement of diffusion within micropatterned PSM, the diffusion and interaction dynamics of individual transmembrane receptors are quantitatively resolved. | |
dc.description.sponsorship | DFGGerman Research Foundation (DFG)European Commission [PI405-4, SFB 944]; European CommunityEuropean Commission [223608]; FP6 Marie-Curie EST fellowship [MEST-CT-2004-504272]; Minerva Foundation; S.W. and F.R. contributed equally to this manuscript. We thank Gabriele Hikade and Hella Kenneweg for technical support. This project was supported by funding from the DFG (PI405-4 and SFB 944) and by the European Community's Seventh Framework Programme (FP7/2007-2013) under grant agreement no 223608 (IFNaction). S.W. was supported by a FP6 Marie-Curie EST fellowship (grant no. MEST-CT-2004-504272) and by a PhD fellowship from the Minerva Foundation. | |
dc.language.iso | en | |
dc.publisher | WILEY-V C H VERLAG GMBH | |
dc.relation.ispartof | SMALL | |
dc.subject | BILAYERS | |
dc.subject | CELL-MEMBRANES | |
dc.subject | Chemistry | |
dc.subject | Chemistry, Multidisciplinary | |
dc.subject | Chemistry, Physical | |
dc.subject | GIANT UNILAMELLAR VESICLES | |
dc.subject | IMMOBILIZATION | |
dc.subject | LATERAL DIFFUSION | |
dc.subject | LIPID-MEMBRANES | |
dc.subject | Materials Science | |
dc.subject | Materials Science, Multidisciplinary | |
dc.subject | membrane proteins | |
dc.subject | MICROSCOPY | |
dc.subject | Nanoscience & Nanotechnology | |
dc.subject | photolithography | |
dc.subject | Physics | |
dc.subject | Physics, Applied | |
dc.subject | Physics, Condensed Matter | |
dc.subject | polymer-supported membranes | |
dc.subject | proteinprotein interactions | |
dc.subject | RECEPTOR INTERACTIONS | |
dc.subject | Science & Technology - Other Topics | |
dc.subject | SINGLE-MOLECULE FLUORESCENCE | |
dc.subject | single-molecule imaging | |
dc.subject | TRACKING | |
dc.title | Diffusion and Interaction Dynamics of Individual Membrane Protein Complexes Confined in Micropatterned Polymer-Supported Membranes | |
dc.type | journal article | |
dc.identifier.doi | 10.1002/smll.201201530 | |
dc.identifier.isi | ISI:000315103300011 | |
dc.description.volume | 9 | |
dc.description.issue | 4 | |
dc.description.startpage | 570 | |
dc.description.endpage | 577 | |
dc.contributor.orcid | 0000-0001-6551-3219 | |
dc.identifier.eissn | 16136829 | |
dc.publisher.place | POSTFACH 101161, 69451 WEINHEIM, GERMANY | |
dcterms.isPartOf.abbreviation | Small | |
crisitem.author.dept | FB 05 - Biologie/Chemie | - |
crisitem.author.deptid | fb05 | - |
crisitem.author.orcid | 0000-0002-2143-2270 | - |
crisitem.author.parentorg | Universität Osnabrück | - |
crisitem.author.netid | PiJa938 | - |
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geprüft am 13.05.2024