ION CHANNELS IN THE THYLAKOID MEMBRANE (A PATCH-CLAMP STUDY)

Autor(en): ENZ, C
STEINKAMP, T
WAGNER, R 
Stichwörter: Biochemistry & Molecular Biology; Biophysics; CHLOROPLAST; CO2 FIXATION; ELECTRON TRANSPORT; INTACT CHLOROPLASTS; ION CHANNEL; LIGHT-DEPENDENT CHANGES; MEMBRANE POTENTIAL; PHOTOPHOSPHORYLATION; STROMAL MG2+ CONCENTRATION; THYLAKOID MEMBRANE
Erscheinungsdatum: 1993
Herausgeber: ELSEVIER SCIENCE BV
Journal: BIOCHIMICA ET BIOPHYSICA ACTA
Volumen: 1143
Ausgabe: 1
Startseite: 67
Seitenende: 76
Zusammenfassung: 
Thylakoid membranes were fused with azolectin liposomes and enlarged to giant thylakoid/liposome vesicles with diameters between 20 mum and 50 mum by a modified dehydration/rehydration procedure. Ion transport across the membrane of these giant thylakoid/liposome vesicles was investigated by the patch-clamp technique. Single-channel currents were observed and four different specific ion conductances could be distinguished. Our results indicate that these conductances can be attributed to three types of ion channel: a putative potassium channel with a conductance of 110 pS (in 100 mM KCl) a chloride channel with a conductance of 220 pS (in 100 mM KCl) and a moderate selectivity of about 4 : 1 for anions over cations. The third type of putative ion channel we observed was permeable to Mg2+ and Ca2+ ions with conductances of 20 pS for Ca2+ and 35 pS for Mg2+, respectively.
ISSN: 00063002
DOI: 10.1016/0005-2728(93)90217-4

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