Spatial Organization of Lipid Phases in Micropatterned Polymer-Supported Membranes

Autor(en): Roder, Friedrich
Birkholz, Oliver
Beutel, Oliver
Paterok, Dirk
Piehler, Jacob 
Stichwörter: ACTIN; Chemistry; Chemistry, Multidisciplinary; DIFFUSION; DOMAIN FORMATION; DYNAMICS; HETEROGENEITY; MICRODOMAINS; MODEL; PROTEINS; RAFTS; RECONSTITUTION
Erscheinungsdatum: 2013
Herausgeber: AMER CHEMICAL SOC
Journal: JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
Volumen: 135
Ausgabe: 4
Startseite: 1189
Seitenende: 1192
Zusammenfassung: 
We have established an approach for the spatial control of lipid phase separation in tethered polymer-supported membranes (PSMs), which were obtained by vesicle fusion on a poly(ethylene glycol) polymer brush functionalized with fatty acid moieties. Phase separation of ternary lipid mixtures (1,2-dioleoyl-sn-glycero-3-phosphocholine/sphingomyelin/cholesterol) into liquid-disordered (l(d)) and liquid-ordered (l(o)) phases within both leaflets was obtained with palmitic acid as the anchoring group. In contrast, tethering of the PSM with oleic acid interfered with the phase separation in the surface-proximal leaflet. We exploited this feature for the assembly of l(o) domains within PSMs into defined structures by binary micropatterning of palmitic and oleic acid into complementary areas. Ternary lipid mixtures spontaneously separated into l(o) and l(d) phases controlled by the geometry of the underlying tethers. Transmembrane proteins reconstituted in these phase-separated PSMs strictly partitioned into the l(d) phase. Hence, the l(o) phase could be used for confining transmembrane proteins into microscopic and submicroscopic domains.
ISSN: 00027863
DOI: 10.1021/ja310186g

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