Thylakoid membranes contain a high-conductance channel

Autor(en): Hinnah, SC
Wagner, R 
Stichwörter: Biochemistry & Molecular Biology; chloroplast; DARK; DRIVEN; fluorescence; INHIBITION; INNER MITOCHONDRIAL-MEMBRANE; INTACT CHLOROPLASTS; ion channel; LIGHT; patch clamp; PATHWAYS; PH; PHOTOPHOSPHORYLATION; thylakoid; TRANSPORT
Erscheinungsdatum: 1998
Volumen: 253
Ausgabe: 3
Startseite: 606
Seitenende: 613
Ion channels in the thylakoid membrane were investigated by direct patch clamping on swollen thylakoids. A preparation method has been developed in order to release osmotically swollen intact thylakoids from pea protoplasts derived from cotyledons of young Pisum sativum plants. The swollen thylakoids with typical diameters between 10 mu m and 20 mu m formed reproducibly high-resistance seals with patch pipettes. We observed a potassium channel with a main conductant state of Lambda approximate to 40 pS and a conductance of Lambda approximate to 90 pS (in asymmetric 20/100 mM KCl) for the fully open channel. Surprisingly, the thylakoid membranes also contained a high-conductance channel with a main conductant slate of Lambda approximate to 620 pS (in asymmetric 20/100 mM KCl), revealing also higher and lower conductant states. With a different experimental approach we showed that thylakoids are able to accumulate transiently the membrane impermeant fluorescent dye Lucifer Yellow which likewise suggests the presence of a pore-like channel with a diameter large enough to allow permeation of Lucifer Yellow.
ISSN: 00142956
DOI: 10.1046/j.1432-1327.1998.2530606.x

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