The mtl genes and the mannitol-l-phosphate dehydrogenase from Klebsiella pneumoniae KAY2026

Autor(en): Otte, S
Lengeler, JW
Stichwörter: BACTERIOPHAGE-T7 RNA-POLYMERASE; CHAIN ALCOHOL DEHYDROGENASES; CLONING; DEPENDENT PHOSPHOTRANSFERASE SYSTEM; ENZYMES; ESCHERICHIA-COLI K-12; EXPRESSION; Klebsiella pneumoniae; mannitol; MANNITOL-1-PHOSPHATE DEHYDROGENASE; mannitol-l-phosphate dehyrogenase; Microbiology; mtl gene; NUCLEOTIDE-SEQUENCE; TRANSPORT
Erscheinungsdatum: 2001
Herausgeber: ELSEVIER SCIENCE BV
Journal: FEMS MICROBIOLOGY LETTERS
Volumen: 194
Ausgabe: 2
Startseite: 221
Seitenende: 227
Zusammenfassung: 
The mtl operon of Klebsiella pneumoniae KAY2076 (formerly Aerobacter aerogenes 1033-5P14) was shown to contain as the promoter-proximal gene mtlA, encoding a D-mannitol-specific enzyme II transporter (IICBA(Mtl)). This gene is followed by mtlD, coding for a mannitol-1-phosphate dehydrogenase (MtlD, 382 amino acid residues), and mtlR (MtlR, 195 amino acid residues) coding for a putative repressor, gene mtlR overlaps the termination codon of mtlD. The DNA and protein sequences are highly similar to the corresponding genes (81%, identical bp) and proteins (79-85% identical amino acids) of Escherichia coli K-12. A truncated form of MtlD lacking the 162 C-terminal amino acid residues still shows 10% dehydrogenase activity which may explain the controversy in the literature concerning the properties of mannitol-phosphate and other medium-length dehydrogenases. (C) 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
ISSN: 03781097
DOI: 10.1111/j.1574-6968.2001.tb09473.x

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