The mtl genes and the mannitol-l-phosphate dehydrogenase from Klebsiella pneumoniae KAY2026
Autor(en): | Otte, S Lengeler, JW |
Stichwörter: | BACTERIOPHAGE-T7 RNA-POLYMERASE; CHAIN ALCOHOL DEHYDROGENASES; CLONING; DEPENDENT PHOSPHOTRANSFERASE SYSTEM; ENZYMES; ESCHERICHIA-COLI K-12; EXPRESSION; Klebsiella pneumoniae; mannitol; MANNITOL-1-PHOSPHATE DEHYDROGENASE; mannitol-l-phosphate dehyrogenase; Microbiology; mtl gene; NUCLEOTIDE-SEQUENCE; TRANSPORT | Erscheinungsdatum: | 2001 | Herausgeber: | ELSEVIER SCIENCE BV | Journal: | FEMS MICROBIOLOGY LETTERS | Volumen: | 194 | Ausgabe: | 2 | Startseite: | 221 | Seitenende: | 227 | Zusammenfassung: | The mtl operon of Klebsiella pneumoniae KAY2076 (formerly Aerobacter aerogenes 1033-5P14) was shown to contain as the promoter-proximal gene mtlA, encoding a D-mannitol-specific enzyme II transporter (IICBA(Mtl)). This gene is followed by mtlD, coding for a mannitol-1-phosphate dehydrogenase (MtlD, 382 amino acid residues), and mtlR (MtlR, 195 amino acid residues) coding for a putative repressor, gene mtlR overlaps the termination codon of mtlD. The DNA and protein sequences are highly similar to the corresponding genes (81%, identical bp) and proteins (79-85% identical amino acids) of Escherichia coli K-12. A truncated form of MtlD lacking the 162 C-terminal amino acid residues still shows 10% dehydrogenase activity which may explain the controversy in the literature concerning the properties of mannitol-phosphate and other medium-length dehydrogenases. (C) 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved. |
ISSN: | 03781097 | DOI: | 10.1111/j.1574-6968.2001.tb09473.x |
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geprüft am 02.06.2024