Specific DNA Duplex Formation at an Artificial Lipid Bilayer: towards a New DNA Biosensor Technology
Autor(en): | Werz, Emma Korneev, Sergei Montilla-Martinez, Malayko Wagner, Richard Hemmler, Roland Walter, Claudius Eisfeld, Joerg Gall, Karsten Rosemeyer, Helmut |
Stichwörter: | Artificial lipid bilayers; Biochemistry & Molecular Biology; Biosensors; Chemistry; Chemistry, Multidisciplinary; DNA; Lipids; Oligonucleotides | Erscheinungsdatum: | 2012 | Herausgeber: | WILEY-V C H VERLAG GMBH | Journal: | CHEMISTRY & BIODIVERSITY | Volumen: | 9 | Ausgabe: | 2 | Startseite: | 272 | Seitenende: | 281 | Zusammenfassung: | A novel technique is described which comprises a base-specific DNA duplex formation at a lipid bilayer?H2O-phase boundary layer. Two different probes of oligonucleotides both carrying a double-tailed lipid at the 5'-terminus were incorporated into stable artificial lipid bilayers separating two compartments (cis/trans-channel) of an optically transparent microfluidic sample carrier with perfusion capabilities. Both the cis- and trans-channels are filled with saline buffer. Injection of a cyanine-5-labeled target DNA sequence, which is complementary to only one of the oligonucleotide probes, into the cis-channel, followed by a thorough perfusion, leads to an immobilization of the labeled complementary oligonucleotide on the membrane as detected by single-molecule fluorescence spectroscopy and microscopy. In the case of fluorescent but non-complementary DNA sequences, no immobilized fluorescent oligonucleotide duplex could be detected on the membrane. This clearly verifies a specific duplex formation at the membrane interface. |
ISSN: | 16121872 | DOI: | 10.1002/cbdv.201100298 |
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geprüft am 17.05.2024