Specific DNA Duplex Formation at an Artificial Lipid Bilayer: towards a New DNA Biosensor Technology

Autor(en): Werz, Emma
Korneev, Sergei 
Montilla-Martinez, Malayko
Wagner, Richard 
Hemmler, Roland
Walter, Claudius
Eisfeld, Joerg
Gall, Karsten
Rosemeyer, Helmut 
Stichwörter: Artificial lipid bilayers; Biochemistry & Molecular Biology; Biosensors; Chemistry; Chemistry, Multidisciplinary; DNA; Lipids; Oligonucleotides
Erscheinungsdatum: 2012
Herausgeber: WILEY-V C H VERLAG GMBH
Journal: CHEMISTRY & BIODIVERSITY
Volumen: 9
Ausgabe: 2
Startseite: 272
Seitenende: 281
Zusammenfassung: 
A novel technique is described which comprises a base-specific DNA duplex formation at a lipid bilayer?H2O-phase boundary layer. Two different probes of oligonucleotides both carrying a double-tailed lipid at the 5'-terminus were incorporated into stable artificial lipid bilayers separating two compartments (cis/trans-channel) of an optically transparent microfluidic sample carrier with perfusion capabilities. Both the cis- and trans-channels are filled with saline buffer. Injection of a cyanine-5-labeled target DNA sequence, which is complementary to only one of the oligonucleotide probes, into the cis-channel, followed by a thorough perfusion, leads to an immobilization of the labeled complementary oligonucleotide on the membrane as detected by single-molecule fluorescence spectroscopy and microscopy. In the case of fluorescent but non-complementary DNA sequences, no immobilized fluorescent oligonucleotide duplex could be detected on the membrane. This clearly verifies a specific duplex formation at the membrane interface.
ISSN: 16121872
DOI: 10.1002/cbdv.201100298

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