TEV protease cleavage in generation of artificial substrate proteins bearing neo-N-termini
Autor(en): | Winckler, Lioba Inken Dissmeyer, Nico |
Stichwörter: | N-degron pathway; Proteases; Protein degradation; TEV | Erscheinungsdatum: | 2023 | Herausgeber: | Academic Press Inc. | Journal: | Methods in Enzymology | Zusammenfassung: | The tobacco etch virus (TEV) protease is widely used in in vitro and in vivo approaches for the removal of affinity tags from fusion proteins or the generation of proteins with a desired N-terminal amino acid. Processing of fusion proteins by the TEV protease can either be achieved by encoding the TEV protease and its recognition site on one construct (self-cleavage) or on two different constructs (co-expression). Here, we compare the efficiency of the self-splitting approach to the co-expression approach. © 2023 Elsevier Inc. |
Beschreibung: | Cited by: 1 |
ISSN: | 0076-6879 | DOI: | 10.1016/bs.mie.2023.02.015 | Externe URL: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85151633999&doi=10.1016%2fbs.mie.2023.02.015&partnerID=40&md5=b5bc9f862ffa1c77d646681df9ab96bb |
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geprüft am 18.05.2024