Functional analysis of ABCG and ABCH transporters from the red flour beetle, Tribolium castaneum
DC Element | Wert | Sprache |
---|---|---|
dc.contributor.author | Rosner, Janin | |
dc.contributor.author | Tietmeyer, Johanne | |
dc.contributor.author | Merzendorfer, Hans | |
dc.date.accessioned | 2021-12-23T16:07:24Z | - |
dc.date.available | 2021-12-23T16:07:24Z | - |
dc.date.issued | 2021 | |
dc.identifier.issn | 1526498X | |
dc.identifier.uri | https://osnascholar.ub.uni-osnabrueck.de/handle/unios/7866 | - |
dc.description.abstract | BACKGROUND ATP-binding cassette transporter (ABC transporter) subfamilies ABCA-C and ABCG-H have been implicated in insecticide detoxification, mostly based on findings of elevated gene expression in response to insecticide treatment. We previously characterized TcABCA-C genes from the model beetle and pest Tribolium castaneum and demonstrated that TcABCA and TcABCC genes are involved in the elimination of diflubenzuron, because RNA interference (RNAi)-mediated gene silencing increased susceptibility. In this study, we focused on the potential functions of TcABCG and TcABCH genes in insecticide detoxification. RESULTS When we silenced the expression of TcABCG-H genes using RNAi, we noticed a previously unreported developmental RNAi phenotype for TcABCG-4F, which is characterized by 50% mortality and ecdysial arrest during adult moult. When we knocked down the Drosophila brown orthologue TcABCG-XC, we did not obtain apparent eye colour phenotypes but did observe a loss of riboflavin uptake by Malpighian tubules. Next, we determined the expression profiles of all TcABCG-H genes in different tissues and developmental stages and analysed transcript levels in response to treatment with four chemically unrelated insecticides. We found that some genes were specifically upregulated after insecticide treatment. However, when we determined insecticide-induced mortalities in larvae that were treated by double-stranded RNA injection to silence those TcABCG-H genes that were upregulated, we did not observe a significant increase in susceptibility to insecticides. CONCLUSION Our findings suggest that the observed insecticide-dependent induction of TcABCG-H gene expression reflects an unspecific stress response, and hence underlines the significance of functional studies on insecticide detoxification. | |
dc.description.sponsorship | Deutsche ForschungsgemeinschaftGerman Research Foundation (DFG) [DFG Me2210/4-1]; We are grateful to Benedikt Wellmeyer for providing cDNA samples of the pu11 strain and commenting on the manuscript, and to Mark A. Hase for his support on the R software package. This work was supported by grants from the Deutsche Forschungsgemeinschaft (DFG Me2210/4-1).; Open access funding enabled and organized by Projekt DEAL. | |
dc.language.iso | en | |
dc.publisher | JOHN WILEY & SONS LTD | |
dc.relation.ispartof | PEST MANAGEMENT SCIENCE | |
dc.subject | ‐ | |
dc.subject | β | |
dc.subject | Agriculture | |
dc.subject | Agronomy | |
dc.subject | ATP‐ | |
dc.subject | binding cassette transporters (ABC transporter) | |
dc.subject | cyfluthrin | |
dc.subject | diflubenzuron | |
dc.subject | Entomology | |
dc.subject | insecticide detoxification | |
dc.subject | malathion | |
dc.subject | tebufenozide | |
dc.subject | Tribolium castaneum | |
dc.title | Functional analysis of ABCG and ABCH transporters from the red flour beetle, Tribolium castaneum | |
dc.type | journal article | |
dc.identifier.doi | 10.1002/ps.6332 | |
dc.identifier.isi | ISI:000625713500001 | |
dc.description.volume | 77 | |
dc.description.issue | 6 | |
dc.description.startpage | 2955 | |
dc.description.endpage | 2963 | |
dc.identifier.eissn | 15264998 | |
dc.publisher.place | THE ATRIUM, SOUTHERN GATE, CHICHESTER PO19 8SQ, W SUSSEX, ENGLAND | |
dcterms.isPartOf.abbreviation | Pest Manag. Sci. | |
dcterms.oaStatus | hybrid | |
crisitem.author.dept | FB 05 - Biologie/Chemie | - |
crisitem.author.deptid | fb05 | - |
crisitem.author.parentorg | Universität Osnabrück | - |
crisitem.author.netid | MeHa731 | - |
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geprüft am 11.05.2024