Evaluation and Elucidation of DNA-Containing Viologen Dendrimer Complex Formation
| Autor(en): | Bongard, Dirk Degefa, Tesfaye Hailu Schon, Peter Giese, Maria Brandt, Roland Walder, Lorenz |
Stichwörter: | AGENTS; ATOMIC-FORCE MICROSCOPY; Chemistry; Chemistry, Organic; Cyclic voltammetry; Dendrimers; Dendriplex formation; DNA; ELECTRON-TRANSFER; GENE DELIVERY; MECHANISM; MOLECULES; PHOSPHORUS DENDRIMERS; POLYMERS; SKELETON; Supramolecular chemistry; TRANSFECTION | Erscheinungsdatum: | 2016 | Herausgeber: | WILEY-V C H VERLAG GMBH | Journal: | EUROPEAN JOURNAL OF ORGANIC CHEMISTRY | Volumen: | 2016 | Ausgabe: | 10 | Startseite: | 1897 | Seitenende: | 1907 | Zusammenfassung: | The syntheses of a tetracationic viologen and polycationic viologen dendrimers are described in this report. These water-soluble viologen dendrimers, the tetracationic viologen, and their DNA-containing complexes have been investigated by cyclic voltammetry (CV), electrophoresis, and atomic force microscopy with respect to their stability and effectiveness as possible transfection systems. The appearance of a negative potential shift and the loss of current in the cyclovoltammograms of viologen dendriplexes have been attributed to DNA/dendrimer complex formation. Oligomers of viologen with at least 4 positive charges are required for the formation of a primary DNA-viologen complex. The dynamics of the formation of a secondary complex was observed and studied by cyclic voltammetry. AFM measurements together with molecular modeling support the electrochemical results and show two different aggregates of the dendriplexes on gold. |
ISSN: | 1434193X | DOI: | 10.1002/ejoc.201501485 |
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