Evaluation and Elucidation of DNA-Containing Viologen Dendrimer Complex Formation

Autor(en): Bongard, Dirk
Degefa, Tesfaye Hailu
Schon, Peter
Giese, Maria
Brandt, Roland 
Walder, Lorenz 
Stichwörter: AGENTS; ATOMIC-FORCE MICROSCOPY; Chemistry; Chemistry, Organic; Cyclic voltammetry; Dendrimers; Dendriplex formation; DNA; ELECTRON-TRANSFER; GENE DELIVERY; MECHANISM; MOLECULES; PHOSPHORUS DENDRIMERS; POLYMERS; SKELETON; Supramolecular chemistry; TRANSFECTION
Erscheinungsdatum: 2016
Herausgeber: WILEY-V C H VERLAG GMBH
Journal: EUROPEAN JOURNAL OF ORGANIC CHEMISTRY
Volumen: 2016
Ausgabe: 10
Startseite: 1897
Seitenende: 1907
Zusammenfassung: 
The syntheses of a tetracationic viologen and polycationic viologen dendrimers are described in this report. These water-soluble viologen dendrimers, the tetracationic viologen, and their DNA-containing complexes have been investigated by cyclic voltammetry (CV), electrophoresis, and atomic force microscopy with respect to their stability and effectiveness as possible transfection systems. The appearance of a negative potential shift and the loss of current in the cyclovoltammograms of viologen dendriplexes have been attributed to DNA/dendrimer complex formation. Oligomers of viologen with at least 4 positive charges are required for the formation of a primary DNA-viologen complex. The dynamics of the formation of a secondary complex was observed and studied by cyclic voltammetry. AFM measurements together with molecular modeling support the electrochemical results and show two different aggregates of the dendriplexes on gold.
ISSN: 1434193X
DOI: 10.1002/ejoc.201501485

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