Fluorescent DNA: the development of 7-deazapurine nucleoside triphosphates applicable for sequencing at the single molecule level

Autor(en): Seela, F
Feiling, E
Gross, J
Hillenkamp, F
Ramzaeva, N
Rosemeyer, H 
Zulauf, M
Stichwörter: 2'-DEOXYRIBOFURANOSIDES; 7-DEAZA-2'-DEOXYGUANOSINES; Biotechnology & Applied Microbiology; CHAINS; DNA polymerases; ion-exchange HPLC; LENGTH; NUCLEIC-ACIDS; nucleoside triphosphates; oligodeoxynucleotides; OLIGONUCLEOTIDES; STABILITY
Erscheinungsdatum: 2001
Herausgeber: ELSEVIER SCIENCE BV
Journal: JOURNAL OF BIOTECHNOLOGY
Volumen: 86
Ausgabe: 3, SI
Startseite: 269
Seitenende: 279
Zusammenfassung: 
7-Deaza-2'-deoxyadenosine and -guanosine phosphoramidite building blocks as well as corresponding 5'-triphosphate derivatives are described carrying in position 7 substituents such as iodo, hexyn-1-yl or 5-aminopentyn-1-yl residues. The phosphoramidites were used to synthesize a series of modified oligodeoxynucleotides. A systematic study of the thermal stabilities of these oligonucleotide duplexes demonstrated that the 7-substituents are well accommodated in the major groove of B-DNA. The 7-(aminoalkyn-1-yl)-7-deazapurine 2'-deoxynucleoside triphosphates were labeled with bulky fluorophores such as Rhodamine Green (R) or tetramethylrhodamine. (C) 2001 Elsevier Science B.V. All rights reserved.
ISSN: 01681656
DOI: 10.1016/S0168-1656(00)00418-1

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