Fluorescent DNA: the development of 7-deazapurine nucleoside triphosphates applicable for sequencing at the single molecule level
Autor(en): | Seela, F Feiling, E Gross, J Hillenkamp, F Ramzaeva, N Rosemeyer, H Zulauf, M |
Stichwörter: | 2'-DEOXYRIBOFURANOSIDES; 7-DEAZA-2'-DEOXYGUANOSINES; Biotechnology & Applied Microbiology; CHAINS; DNA polymerases; ion-exchange HPLC; LENGTH; NUCLEIC-ACIDS; nucleoside triphosphates; oligodeoxynucleotides; OLIGONUCLEOTIDES; STABILITY | Erscheinungsdatum: | 2001 | Herausgeber: | ELSEVIER SCIENCE BV | Journal: | JOURNAL OF BIOTECHNOLOGY | Volumen: | 86 | Ausgabe: | 3, SI | Startseite: | 269 | Seitenende: | 279 | Zusammenfassung: | 7-Deaza-2'-deoxyadenosine and -guanosine phosphoramidite building blocks as well as corresponding 5'-triphosphate derivatives are described carrying in position 7 substituents such as iodo, hexyn-1-yl or 5-aminopentyn-1-yl residues. The phosphoramidites were used to synthesize a series of modified oligodeoxynucleotides. A systematic study of the thermal stabilities of these oligonucleotide duplexes demonstrated that the 7-substituents are well accommodated in the major groove of B-DNA. The 7-(aminoalkyn-1-yl)-7-deazapurine 2'-deoxynucleoside triphosphates were labeled with bulky fluorophores such as Rhodamine Green (R) or tetramethylrhodamine. (C) 2001 Elsevier Science B.V. All rights reserved. |
ISSN: | 01681656 | DOI: | 10.1016/S0168-1656(00)00418-1 |
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