Magnetogenetic Control of Protein Gradients Inside Living Cells with High Spatial and Temporal Resolution
Autor(en): | Etoc, Fred Vicario, Chiara Lisse, Domenik Siaugue, Jean-Michel Piehler, Jacob Coppey, Mathieu Dahan, Maxime |
Stichwörter: | cell signaling; Chemistry; Chemistry, Multidisciplinary; Chemistry, Physical; intracellular diffusion; LIGHT; LIVE CELLS; MACROMOLECULES; MAGNETIC NANOPARTICLES; Magnetogenetics; Materials Science; Materials Science, Multidisciplinary; MOLECULES; nanomagnetism; nanoparticles; Nanoscience & Nanotechnology; Physics; Physics, Applied; Physics, Condensed Matter; protein manipulation; Science & Technology - Other Topics; SPATIOTEMPORAL CONTROL; SYSTEMS; VELOCITY | Erscheinungsdatum: | 2015 | Herausgeber: | AMER CHEMICAL SOC | Journal: | NANO LETTERS | Volumen: | 15 | Ausgabe: | 5 | Startseite: | 3487 | Seitenende: | 3494 | Zusammenfassung: | Tools for controlling the spatial organization of proteins are a major prerequisite for deciphering mechanisms governing the dynamic architecture of living cells. Here, we have developed a generic approach for inducing and maintaining protein gradients inside living cells by meaty of biofunctionalized magnetic nanoparticles (MNPs). For this purpose; we tailored the size and surface properties of MNPs in order to ensure unhindered mobility in the cytosol. These MNPs with a core diameter below 50 nm could be rapidly relocalized in living cells by exploiting biased diffusion at weak magnetic forces in the femto-Newton range. In combination with MNP surface functionalization for specific in situ capturing of target proteins as well as efficient delivery into the cytosplasm, we here present a comprehensive technology for controlling intracellular protein gradients with a temporal resolution of a few tens of seconds. |
ISSN: | 15306984 | DOI: | 10.1021/acs.nanolett.5b00851 |
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geprüft am 28.04.2024