Differential Activity of Type I Interferon Subtypes for Dendritic Cell Differentiation
Autor(en): | Garcin, Genevieve Bordat, Yann Chuchana, Paul Monneron, Daniele Law, Helen K. W. Piehler, Jacob Uze, Gilles |
Stichwörter: | ACTIVATION; APOPTOTIC CELLS; CD8(+) T-CELLS; COMPLEMENT RECEPTOR; EXPRESSION; FUNCTIONAL-ACTIVITIES; IFN-ALPHA; Multidisciplinary Sciences; PBL-SCID MICE; PHAGOCYTOSIS; Science & Technology - Other Topics; TOLERANCE | Erscheinungsdatum: | 2013 | Herausgeber: | PUBLIC LIBRARY SCIENCE | Journal: | PLOS ONE | Volumen: | 8 | Ausgabe: | 3 | Zusammenfassung: | The type I interferon (IFN) family comprises 15 cytokines (in human 13 alpha, 1 beta, 1 omega), which exert several cellular functions through binding to a common receptor. Despite initial activation of the same Jak/Stat signalling pathway, the cellular response may differ depending on type I IFN subtype. We investigated the activity of six type I IFN subtypes -IFN alpha 1, alpha 2, alpha 8, alpha 21, omega and beta- to promote the differentiation of dendritic cells (DC). Transcriptome analyses identified two distinct groups, the IFN alpha/omega-DC and the IFN beta-DC. In addition, the expression level of seven chemokines and several cell surface markers characteristic of DC distinguished IFN alpha-DC and IFN beta-DC. These differences are unlikely to impact the efficacy of T cell functional response since IFN alpha 2-DC and IFN beta-DC were equipotent in inducing the proliferation and the polarization of allogenic naive CD4 T cells into Th1 cells and in stimulating autologous antigen specific CD4 or CD8 T cells. Of the functional parameters analysed, the only one that showed a modest differential was the phagocytic uptake of dead cells which was higher for IFN alpha 2-DC. |
ISSN: | 19326203 | DOI: | 10.1371/journal.pone.0058465 |
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geprüft am 15.05.2024