High-Affinity Labeling and Tracking of Individual Histidine-Tagged Proteins in Live Cells Using Ni2+ Tris-nitrilotriacetic Acid Quantum Dot Conjugates
| Autor(en): | Roullier, Victor Clarke, Samuel You, Changjiang Pinaud, Fabien Gouzer, Geraldine Schaible, Dirk Marchi-Artzner, Valerie Piehler, Jacob Dahan, Maxime |
Stichwörter: | BIOTIN LIGASE; CDSE/ZNS NANOCRYSTALS; Chemistry; Chemistry, Multidisciplinary; Chemistry, Physical; DIFFUSION; IMMOBILIZATION; LIVING CELLS; Materials Science; Materials Science, Multidisciplinary; MEMBRANE; Nanoscience & Nanotechnology; PEPTIDES; Physics; Physics, Applied; Physics, Condensed Matter; POLYHISTIDINE SEQUENCES; RECEPTOR INTERACTIONS; Science & Technology - Other Topics; SURFACE-PROTEINS | Erscheinungsdatum: | 2009 | Herausgeber: | AMER CHEMICAL SOC | Journal: | NANO LETTERS | Volumen: | 9 | Ausgabe: | 3 | Startseite: | 1228 | Seitenende: | 1234 | Zusammenfassung: | Investigation of many cellular processes using fluorescent quantum dots (QDs) is hindered by the nontrivial requirements for OD surface functionalization and targeting. To address these challenges, we designed, characterized and applied QD-trisNTA, which integrates trisnitrilotriacetic acid, a small and high-affinity recognition unit for the ubiquitous polyhistidine protein tag. Using GD-trisNTA, we demonstrate two-color OD tracking of the type-1 interferon receptor subunits in live cells, potentially enabling direct visualization of protein-protein interactions at the single molecule level. |
ISSN: | 15306984 | DOI: | 10.1021/nl9001298 |
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geprüft am 26.04.2024
