High-Affinity Labeling and Tracking of Individual Histidine-Tagged Proteins in Live Cells Using Ni2+ Tris-nitrilotriacetic Acid Quantum Dot Conjugates

Autor(en): Roullier, Victor
Clarke, Samuel
You, Changjiang 
Pinaud, Fabien
Gouzer, Geraldine
Schaible, Dirk
Marchi-Artzner, Valerie
Piehler, Jacob 
Dahan, Maxime
Stichwörter: BIOTIN LIGASE; CDSE/ZNS NANOCRYSTALS; Chemistry; Chemistry, Multidisciplinary; Chemistry, Physical; DIFFUSION; IMMOBILIZATION; LIVING CELLS; Materials Science; Materials Science, Multidisciplinary; MEMBRANE; Nanoscience & Nanotechnology; PEPTIDES; Physics; Physics, Applied; Physics, Condensed Matter; POLYHISTIDINE SEQUENCES; RECEPTOR INTERACTIONS; Science & Technology - Other Topics; SURFACE-PROTEINS
Erscheinungsdatum: 2009
Herausgeber: AMER CHEMICAL SOC
Journal: NANO LETTERS
Volumen: 9
Ausgabe: 3
Startseite: 1228
Seitenende: 1234
Zusammenfassung: 
Investigation of many cellular processes using fluorescent quantum dots (QDs) is hindered by the nontrivial requirements for OD surface functionalization and targeting. To address these challenges, we designed, characterized and applied QD-trisNTA, which integrates trisnitrilotriacetic acid, a small and high-affinity recognition unit for the ubiquitous polyhistidine protein tag. Using GD-trisNTA, we demonstrate two-color OD tracking of the type-1 interferon receptor subunits in live cells, potentially enabling direct visualization of protein-protein interactions at the single molecule level.
ISSN: 15306984
DOI: 10.1021/nl9001298

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