Monofunctional Stealth Nanoparticle for Unbiased Single Molecule Tracking Inside Living Cells
Autor(en): | Lisse, Domenik Richter, Christian P. Drees, Christoph Birkholz, Oliver You, Changjiang Rampazzo, Enrico Piehler, Jacob |
Stichwörter: | AUTOPHAGY; BINDING; BIOMOLECULES; Chemistry; Chemistry, Multidisciplinary; Chemistry, Physical; cytosol; DENSITY; DIFFUSION; IMPACT; Materials Science; Materials Science, Multidisciplinary; MEMBRANES; mitochondria; monofunctionalization; Nanoscience & Nanotechnology; Physics; Physics, Applied; Physics, Condensed Matter; PROTEIN CORONA; PROVIDE; QUANTUM DOTS; Science & Technology - Other Topics; single particle tracking; Stealth nanoparticle | Erscheinungsdatum: | 2014 | Herausgeber: | AMER CHEMICAL SOC | Journal: | NANO LETTERS | Volumen: | 14 | Ausgabe: | 4 | Startseite: | 2189 | Seitenende: | 2195 | Zusammenfassung: | On the basis of a protein cage scaffold, we have systematically explored intracellular application of nanoparticles for single molecule studies and discovered that recognition by the autophagy machinery plays a key role for rapid metabolism in the cytosol. Intracellular stealth nanoparticles were achieved by heavy surface PEGylation. By combination with a generic approach for nanoparticle monofunctionalization, efficient labeling of intracellular proteins with high fidelity was accomplished, allowing unbiased long-term tracking of proteins in the outer mitochondrial membrane. |
ISSN: | 15306984 | DOI: | 10.1021/nl500637a |
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geprüft am 28.04.2024