Monofunctional Stealth Nanoparticle for Unbiased Single Molecule Tracking Inside Living Cells

Autor(en): Lisse, Domenik
Richter, Christian P.
Drees, Christoph
Birkholz, Oliver
You, Changjiang 
Rampazzo, Enrico
Piehler, Jacob 
Stichwörter: AUTOPHAGY; BINDING; BIOMOLECULES; Chemistry; Chemistry, Multidisciplinary; Chemistry, Physical; cytosol; DENSITY; DIFFUSION; IMPACT; Materials Science; Materials Science, Multidisciplinary; MEMBRANES; mitochondria; monofunctionalization; Nanoscience & Nanotechnology; Physics; Physics, Applied; Physics, Condensed Matter; PROTEIN CORONA; PROVIDE; QUANTUM DOTS; Science & Technology - Other Topics; single particle tracking; Stealth nanoparticle
Erscheinungsdatum: 2014
Herausgeber: AMER CHEMICAL SOC
Journal: NANO LETTERS
Volumen: 14
Ausgabe: 4
Startseite: 2189
Seitenende: 2195
Zusammenfassung: 
On the basis of a protein cage scaffold, we have systematically explored intracellular application of nanoparticles for single molecule studies and discovered that recognition by the autophagy machinery plays a key role for rapid metabolism in the cytosol. Intracellular stealth nanoparticles were achieved by heavy surface PEGylation. By combination with a generic approach for nanoparticle monofunctionalization, efficient labeling of intracellular proteins with high fidelity was accomplished, allowing unbiased long-term tracking of proteins in the outer mitochondrial membrane.
ISSN: 15306984
DOI: 10.1021/nl500637a

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